Elucidating the Mode of Action of Hybrid Nanoparticles of Cu/Zn against Copper Tolerant Xanthomonas euvesicatoria

The widespread presence of tolerance to copper in Xanthomonas species has resulted in the need to develop alternative approaches to control plant diseases caused by xanthomonads. In recent years, nanotechnological approaches have resulted in the identification of novel materials to control plant pathogens. While many metal-based nanomaterials have shown promise for disease control, an important question relates to the mode of action of these new materials. In this study we used several approaches such as SEM, propidium monoazide qPCR, epifluorescence microscopy and RNA sequencing to elucidate the mode of action of a Cu/Zn hybrid nanoparticle against copper tolerant strains of Xanthomonas euvesicatoria. We demonstrate that Cu/Zn, unlike Kocide 3000, did not activate copper resistance genes (i.e. copA and copB) in the copper-tolerant bacterium, but functioned by disrupting the bacterial cell structure and perturbing important biological processes such as cell respiration and chemical homeostasis.