Chromosome-scale genome assembly of the 'Munstead' cultivar of Lavandula angustifolia

John P Hamilton; Brieanne Vaillancourt; Joshua C Wood; Haiyan Wang; Jiming Jiang; Douglas E Soltis; C Robin Buell; Pamela S Soltis

doi:10.1186/s12863-023-01181-y

Chromosome-scale genome assembly of the 'Munstead' cultivar of Lavandula angustifolia

BMC Genom Data. 2023 Dec 13;24(1):75. doi: 10.1186/s12863-023-01181-y.

Authors

John P Hamilton^{1

2}, Brieanne Vaillancourt¹, Joshua C Wood¹, Haiyan Wang³, Jiming Jiang^{3

4}, Douglas E Soltis^{5

6}, C Robin Buell^{7

8

9}, Pamela S Soltis^{10

11}

Affiliations

¹ Center for Applied Genetic Technologies, University of Georgia, Athens, GA, USA.
² Department of Crop and Soil Sciences, University of Georgia, Athens, GA, USA.
³ Department of Plant Biology, Michigan State University, East Lansing, MI, USA.
⁴ Department of Horticulture, Michigan State University, East Lansing, MI, USA.
⁵ Department of Biology, University of Florida, Gainesville, FL, USA. dsoltis@ufl.edu.
⁶ Museum of Natural History, University of Florida, Gainesville, FL, USA. dsoltis@ufl.edu.
⁷ Center for Applied Genetic Technologies, University of Georgia, Athens, GA, USA. Robin.Buell@uga.edu.
⁸ Department of Crop and Soil Sciences, University of Georgia, Athens, GA, USA. Robin.Buell@uga.edu.
⁹ Institute of Plant Breeding, Genetics, and Genomics, University of Georgia, Athens, GA, USA. Robin.Buell@uga.edu.
¹⁰ Department of Biology, University of Florida, Gainesville, FL, USA. psoltis@flmnh.ufl.edu.
¹¹ Museum of Natural History, University of Florida, Gainesville, FL, USA. psoltis@flmnh.ufl.edu.

Abstract

Objectives: Lavandula angustifolia (English lavender) is commercially important not only as an ornamental species but also as a major source of fragrances. To better understand the genomic basis of chemical diversity in lavender, we sequenced, assembled, and annotated the 'Munstead' cultivar of L. angustifolia.

Data description: A total of 80 Gb of Oxford Nanopore Technologies reads was used to assemble the 'Munstead' genome using the Canu genome assembler software. Following multiple rounds of error correction and scaffolding using Hi-C data, the final chromosome-scale assembly represents 795,075,733 bp across 25 chromosomes with an N50 scaffold length of 31,371,815 bp. Benchmarking Universal Single Copy Orthologs analysis revealed 98.0% complete orthologs, indicative of a high-quality assembly representative of genic space. Annotation of protein-coding sequences revealed 58,702 high-confidence genes encoding 88,528 gene models. Access to the 'Munstead' genome will permit comparative analyses within and among lavender accessions and provides a pivotal species for comparative analyses within Lamiaceae.

Keywords: Essential oil; Lamiaceae; Terpene.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes
Genome
Genomics
Lavandula* / genetics
Open Reading Frames

Abstract

Publication types

MeSH terms

Grants and funding