Establishment of bovine trophoblast stem cells

Cell Rep. 2023 May 30;42(5):112439. doi: 10.1016/j.celrep.2023.112439. Epub 2023 May 4.

Abstract

Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.

Keywords: CP: Stem cell research; blastocyst; bovine; pluripotency; trophectoderm; trophoblast differentiation; trophoblast stem cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Differentiation / genetics
  • Female
  • Humans
  • Mice
  • Placentation
  • Pluripotent Stem Cells*
  • Pregnancy
  • Trophoblasts*