Labeling and analyzing lipid droplets in mouse muscle stem cells

Jingjuan Chen; Feng Yue; Shihuan Kuang

doi:10.1016/j.xpro.2022.101849

Labeling and analyzing lipid droplets in mouse muscle stem cells

STAR Protoc. 2022 Dec 16;3(4):101849. doi: 10.1016/j.xpro.2022.101849. Epub 2022 Nov 19.

Authors

Jingjuan Chen¹, Feng Yue², Shihuan Kuang³

Affiliations

¹ Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA. Electronic address: chen2627@purdue.edu.
² Department of Animal Sciences, University of Florida, Gainesville, FL 32608, USA.
³ Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA. Electronic address: skuang@purdue.edu.

Abstract

Lipid droplets are emerging as an important and dynamic organelle whose metabolism controls stem cell behavior. Here we present a comprehensive protocol to visualize and quantify these organelles in mouse muscle satellite cells (MuSCs). This protocol includes steps for BODIPY/LipidSpot610 staining of freshly isolated MuSCs, in vitro cultured myoblasts, and single myofibers to label lipid droplets and subsequent analysis and quantification of fluorescence signals. This protocol can be modified to stain lipid droplets in other cell types of interest. For complete details on the use and execution of this protocol, please refer to Yue et al. (2022).¹.

Keywords: Cell Biology; Cell culture; Flow Cytometry/Mass Cytometry; Microscopy; Molecular/Chemical Probes; Stem Cells.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Fluorescence
Lipid Droplets*
Mice
Muscle Fibers, Skeletal
Muscle, Skeletal*
Stem Cells

Abstract

Publication types

MeSH terms

Grants and funding